What is Circular RNA?

• Circular RNAs are covalently closed single-stranded RNA, naturally produced via back-splicing of pre-mRNA exon(s) in eukaryotic cells

• Broadly expressed in metazoans, but generally at low levels with cell/tissue-specific patterns

• Inherent stability with resistance to known exonucleases

• Can be engineered and produced in vitro via in vitro transcription and circularization